Analytical Data
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基因名
Lgr5/GPR49
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简介
The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Human (CHO, hFc) is the recombinant human-derived Lgr5/GPR49 protein, expressed by CHO , with C-hFc labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human R-Spondin 1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5. The ED50 for this effect is 42.14 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human R-Spondin 1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5.The ED50 for this effect is 42.14 ng/mL.
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种属
Human
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表达系统
CHO
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标签
C-hFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75473
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表达区间
G22-I560
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氨基酸序列
GSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLI
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蛋白长度
Partial
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分子量
88-110 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Lgr5, also known as GPR49, is a member of the leucine-rich repeat-containing G protein-coupled receptor family and has garnered significant attention for its role in various biological processes, particularly in stem cell biology and tumorigenesis. Initially identified as a marker for intestinal stem cells, Lgr5 has been shown to play a critical role in maintaining the stem cell niche and regulating cellular proliferation and differentiation. Its interaction with the Wnt signaling pathway suggests that Lgr5 might be pivotal in both normal and malignant tissue homeostasis. Researchers have been exploring Lgr5's potential as a therapeutic target, given its overexpression in several cancers, including colorectal cancer. By studying recombinant Lgr5/GPR49 proteins, scientists aim to elucidate its functional mechanisms and interactions at the molecular level. These investigations not only provide insights into the fundamental biology of stem cells and cancer but also pave the way for developing novel strategies for targeted therapies that can inhibit cancer progression and improve patient outcomes. The production and characterization of Lgr5/GPR49 recombinant proteins facilitate the exploration of its structure-function relationship, aiding in the identification of small molecules or antibodies that could modulate its activity in therapeutic contexts. Overall, the study of Lgr5/GPR49 holds promise for advancing our understanding of stem cell dynamics and cancer biology, addressing critical oncology challenges.
