Analytical Data
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Gene name
PIGL
- Application
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Alternative Names
N-Acetylglucosaminylphosphatidylinositol Deacetylase; Phosphatidylinositol Glycan Anchor Biosynthesis,Class L; N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y2B2
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Expression Region
Met1~Leu252
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Molecular Weight
29kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIGL (Phosphatidylinositol glycan anchor biosynthesis class L) is an essential enzyme involved in the glycosylphosphatidylinositol (GPI) anchor biosynthesis pathway, which is crucial for the post-translational modification of various glycoproteins on the cell surface. GPI anchors play significant roles in cellular processes, including cell signaling, adhesion, and immune responses. Defects in GPI anchor synthesis can lead to a range of disorders, including neurodegenerative diseases and immunodeficiencies. Recent studies have highlighted the importance of PIGL in the assembly of GPI anchors and its potential role in disease mechanisms. Understanding the structure and function of PIGL is vital for elucidating the biochemical pathways that govern GPI anchor biosynthesis and for developing therapeutic strategies for related disorders. Recombinant PIGL protein is generated to study its enzymatic activity, substrate specificity, and interaction with other proteins in the GPI anchor synthesis pathway. These studies aim to provide insights into the functional implications of PIGL in cellular processes and its involvement in pathophysiological conditions, paving the way for targeted interventions in diseases linked to GPI anchor deficiencies.
