Analytical Data
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Gene name
SPTLC1
- Application
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Alternative Names
HSAN; HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI; Hereditary Sensory Neuropathy,Type 1; Long chain base biosynthesis protein 1; Serine-palmitoyl-CoA transferase 1
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O35704
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Expression Region
Asp143~Leu473
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Molecular Weight
40kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPTLC1, or serine palmitoyltransferase long chain base subunit 1, is a key enzyme in the de novo biosynthesis of sphingolipids, which are essential components of cellular membranes and play critical roles in cell signaling and metabolism. Mutations in the SPTLC1 gene have been linked to various disorders, including hereditary sensory neuropathy type 1 (HSN1) and other sphingolipid metabolism-related diseases. The study of SPTLC1 recombinant protein is crucial for understanding its structural and functional characteristics, as well as the molecular mechanisms underlying these diseases. By producing SPTLC1 in a recombinant form, researchers can investigate its enzymatic activity, substrate specificity, and interaction with other proteins involved in sphingolipid metabolism. Furthermore, recombinant SPTLC1 can serve as a valuable tool for drug discovery, allowing for the screening of small molecules that may modulate its activity and provide therapeutic avenues for treating related neuropathies. Advances in recombinant DNA technology and protein expression systems have facilitated the production of functional SPTLC1, thereby enhancing our ability to dissect its biochemical pathways and explore potential interventions for disorders arising from its dysregulation. Overall, research on SPTLC1 recombinant protein holds promise for elucidating the complexities of sphingolipid metabolism and its impact on human health.
