Analytical Data
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基因名
FGF-4
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简介
FGF-4 Protein is an important member of the fibroblast growth factor (FGF) family. By binding to FGFRs (especially FGFR1 and FGFR2), FGF-4 Protein can activate downstream signaling pathways such as RAS-MAPK and PI3K-AKT. FGF-4 Protein plays a key role in physiological processes such as cell growth, differentiation, migration, and angiogenesis. FGF-4 Protein, Human is a recombinant FGF-4 protein expressed by E. coli without a tag[1][2][3].
- Application
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生物活性
The ED50 is ≤1.5 ng/mL as measured by 3T3 cells, corresponding to a specific activity of ≥6.67 × 105 units/mg. Measured in a cell proliferation assay using NIH/3T3 cells. The ED50for this effect is 1.38 ng/mL, corresponding to a specific activity is 7.246×105 units/mg.
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别名
rHuFGF-4; HBGF-4; HST; HST-1; HSTF1
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
P08620-1
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表达区间
A31-L206
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蛋白长度
Full Length of Isoform-1 Mature Protein
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分子量
19-24 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fibroblast Growth Factor 4 (FGF-4) is a crucial member of the fibroblast growth factor family, which plays significant roles in embryonic development, tissue repair, and various physiological processes. Research on FGF-4 has garnered attention due to its involvement in cell proliferation, differentiation, and angiogenesis. Its expression is particularly high during early developmental stages, suggesting a key role in mesodermal and ectodermal patterning. Additionally, FGF-4 has been implicated in tumorigenesis, as abnormally elevated levels are often found in certain cancers. The recombinant protein form of FGF-4 has been developed for both in vitro and in vivo studies, enabling scientists to explore its functional properties and therapeutic potential. Investigations into FGF-4's signaling mechanisms and interactions with specific receptors have revealed insights into its biological activities, which may lead to novel therapeutic strategies for tissue regeneration and cancer treatment. Furthermore, the production of FGF-4 as a recombinant protein facilitates research on its pharmacokinetics and potential use in regenerative medicine, thus highlighting its significance in both basic biological research and clinical applications.
