Analytical Data
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Gene name
Apolipoprotein E/APOE4
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简介
Apolipoprotein E (APOE) plays a key role in lipoprotein-mediated lipid transport and is a core component in the production, transformation, and clearance of plasma lipoproteins. As an amphipathic molecule, APOE binds to various lipoprotein particles, including chylomicrons, chylomicron remnants, VLDL, and IDL, favoring HDL. Apolipoprotein E/APOE Protein, Human (C130R, HEK293, Fc) is the recombinant human-derived Apolipoprotein E/APOE protein, expressed by HEK293 , with N-hFc labeled tag and C130R mutation.
- Application
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Alternative Names
Apolipoprotein E; Apo-E; APOE; apolipo E; APOE4
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Species
Human
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02649
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Expression Region
K19-H317, C130R
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Protein Length
Full Length of Mature Protein
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Molecular Weight
63-68 kDa (Due to glycosylation)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Apolipoprotein E (ApoE) is a critical protein involved in lipid metabolism and neuronal repair processes. It exists in three major isoforms: ApoE2, ApoE3, and ApoE4. The ApoE4 allele has been identified as the primary genetic risk factor for late-onset Alzheimer's disease (AD), with individuals carrying one or two copies of this allele exhibiting a significantly increased risk of developing cognitive decline and neurodegeneration. Research into the recombinant production of ApoE4 protein aims to elucidate its structure-function relationships, understand the molecular mechanisms underlying its association with AD, and explore potential therapeutic targets. As the pathogenic mechanisms associated with ApoE4 are complex, including impaired amyloid plaque clearance and altered neuronal signaling, studying the recombinant protein can provide insights into how these processes differ from those mediated by the beneficial ApoE2 and neutral ApoE3 isoforms. Furthermore, understanding the role of ApoE4 in lipid transport and neuroinflammation is essential for developing targeted interventions. The production of recombinant ApoE4, coupled with advanced biochemical and neurobiological assays, can enhance our knowledge of its functional implications and pave the way for innovative strategies to mitigate AD risk and progression.
