Analytical Data
-
Gene name
HCC-4/CCL16
-
简介
HCC-4/CCL16 Protein, Human (CHO) is a CC chemokine that specifically attracts lymphocytes, dendritic cells and monocytes, increases their adhesion and has myelosuppressive activity. HCC-4/CCL16 Protein, Human (CHO) interacts with CCR1, CCR2, CCR5 and CCR8 to mediate inflammatory and cancer responses. HCC-4/CCL16 Protein, Human (CHO) is a recombinant human HCC-4/CCL16 (Q24-Q120) expressed by CHO[1].
- Application
-
Biological Activity
1.The ED50 is <1.5 μg/mL as measured by CHO-K1/Gα15/hCCR1 cells (human Gα15 and human CCR1 stably expressed in CHO-K1 cells). 2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 1.926 μg/mL, corresponding to a specific activity is 519.211 U/mg. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 1.926 μg/mL, corresponding to a specific activity is 519.211 U/mg.
-
Alternative Names
rHuHCC-4/CCL16; C-C motif chemokine 16; SCYA16
-
Species
Human
-
Source
CHO
-
Tag
Tag Free
-
Purity
Greater than 95% as determined by reducing SDS-PAGE.
-
Uniprot
O15467
-
Expression Region
Q24-Q120
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
12 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HCC-4/CCL16, a chemokine protein, plays a crucial role in the immune response by mediating the recruitment of immune cells to sites of inflammation and infection. It belongs to the CC chemokine family and is primarily expressed in the thymus, lymph nodes, and various tissues during inflammatory conditions. Studies have shown that HCC-4/CCL16 is involved in various pathological processes, including autoimmune diseases, cancer, and chronic inflammatory diseases. Its ability to attract specific subsets of T cells and other immune cells makes it a potential target for therapeutic strategies aimed at modulating immune responses. Recent research has focused on the recombinant expression and purification of HCC-4/CCL16 to explore its biological functions and therapeutic potential. Understanding the structure-functional relationship of HCC-4/CCL16 is essential for developing novel immunotherapeutic approaches. Efforts have been made to characterize its signaling pathways and interactions with receptors, providing insights into its role in human health and disease. The advancements in recombinant protein technology facilitate the generation of sufficient quantities of HCC-4/CCL16 for these studies, paving the way for future investigations into its role as a biomarker or therapeutic agent in various immune-related conditions.
