Cat: IPD-X39091

Recombinant Human TAS1R3 Protein,His

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Analytical Data

  • Gene name

    TAS1R3

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    T1R3; Sweet taste receptor T1R3

  • Species

    Human

  • Source

    E. coli

  • Tag

    N-His

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q7RTX0

  • Expression Region

    Ala21~Leu570

  • Molecular Weight

    65kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

The TAS1R3 gene encodes one of the subunits of the sweet taste receptor, which plays a crucial role in the perception of sweetness and is part of the larger TAS1R family of G protein-coupled receptors (GPCRs). This receptor is widely studied not only for its significance in taste biology but also for its broader implications in metabolic regulation and energy homeostasis. Alterations in TAS1R3 function have been linked to various health issues, including obesity and diabetes, as sweetness perception is closely tied to dietary choices. Research on TAS1R3 recombinant protein involves expressing and purifying this protein to better understand its structural and functional characteristics. By studying the receptor's interaction with different sweet substances and its signal transduction mechanisms, researchers aim to elucidate how sweet taste perception affects feeding behavior and metabolism. Furthermore, the recombinant protein serves as a valuable tool in drug discovery and nutritional science, potentially leading to the development of novel therapeutic strategies targeting metabolic disorders. Overall, studies on TAS1R3 recombinant protein contribute to a deeper understanding of taste signaling pathways and their implications for human health.

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