Analytical Data
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Gene name
HCC-1/CCL14
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简介
HCC-1/CCL14 Protein, Human is a CC chemokine with weak activity against human monocytes, promotes monocyte, eosinophil and T-lymphocyte chemotaxis, and mediates allergic airway inflammation and cancer. HCC-1/CCL14 Protein, Human is a recombinant human HCC-1/CCL14(T22-N93) expressed by E. coli[1][2].
- Application
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Biological Activity
1.Fully biologically active when compared to standard. The biological activity deter mined by a chemotaxis bloassay using human monocytes is in a concentration of 5.0-20 ng/mL. 2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 this effect is 4.297 ng/mL, corresponding to a specific activity is 2.33×105 U/mg. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 4.297 ng/mL, corresponding to a specific activity is 2.33×105 U/mg.
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Alternative Names
rHuHCC-1/CCL14; C-C motif chemokine 14; SCYA14
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
Q16627-1
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Expression Region
T22-N93
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
8-10 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HCC-1/CCL14 is a member of the CC chemokine family, known for its role in mediating immune responses and inflammation. It is primarily expressed in various tissues, including lymphoid organs, and plays a significant role in the recruitment and activation of immune cells, particularly lymphocytes and monocytes. The understanding of HCC-1/CCL14's functions has garnered interest due to its potential implications in various pathological conditions, including cancer, autoimmune diseases, and inflammatory disorders. Research has shown that HCC-1/CCL14 can influence the tumor microenvironment and modulate antitumor immunity, making it a target of investigation for therapeutic strategies. Additionally, studies have highlighted its role in tissue homeostasis and repair mechanisms following injury. The recombinant expression of HCC-1/CCL14 proteins allows for detailed studies into their biological functions, receptor interactions, and signaling pathways. This research may ultimately contribute to the development of novel treatments that harness the immune system to combat diseases, emphasizing the importance of understanding the molecular mechanisms of chemokines like HCC-1/CCL14 in health and disease.
