Analytical Data
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Gene name
UBE2L3
- Application
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Alternative Names
L-UBC; E2-F1; UBCH7; UbcM4; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P68036
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Expression Region
Ser4~Asp154
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Molecular Weight
21kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBE2L3, a member of the ubiquitin-conjugating enzyme (E2) family, plays a pivotal role in the ubiquitination process, which is essential for various cellular functions, including protein degradation, cell cycle regulation, and DNA repair. UBE2L3 has garnered significant attention due to its involvement in diverse pathological processes, including cancer progression, immune responses, and neurodegenerative diseases. Studies have indicated that UBE2L3 may influence the stability and activity of multiple substrates, thereby affecting signaling pathways and cellular homeostasis. Moreover, it has been proposed as a potential therapeutic target, as its overexpression in certain cancers correlates with poor prognosis. Understanding the functional mechanisms of UBE2L3 through recombinant protein studies can provide insights into its role in disease mechanisms and aid in the development of targeted therapies. The production of UBE2L3 as a recombinant protein allows researchers to investigate its biochemical properties, substrate specificity, and interaction with other cellular components in detail. Overall, UBE2L3 holds promise not only for elucidating fundamental biological processes but also for paving the way towards innovative treatment strategies in various diseases.
