Analytical Data
-
Gene name
HIP2
- Application
-
Alternative Names
HYPG; LIG; UBE2K; Ubiquitin-Conjugating Enzyme E2K; Ubiquitin carrier protein; Ubiquitin-protein ligase
-
Species
Human
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P61086
-
Expression Region
Ala2~Asn200
-
Molecular Weight
26kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HIP2 (also known as UBE2J1) is a member of the ubiquitin-conjugating enzyme family, playing a critical role in the ubiquitin-proteasome system, which is essential for protein degradation, regulation of cell cycle, and maintenance of cellular homeostasis. Research into HIP2 has garnered attention due to its involvement in various cellular processes and its implications in cancer biology and neurodegenerative diseases. Studies have shown that aberrations in ubiquitination pathways can lead to pathological states, making HIP2 a potential therapeutic target. The functional characterization of HIP2 through recombinant protein studies enables researchers to unravel its specific role in ubiquitin-related pathways, elucidating the mechanisms by which it influences protein turnover and cellular signaling. Furthermore, recombinant HIP2 proteins can serve as valuable tools in drug discovery, providing insights into novel strategies for modulating the ubiquitin-proteasome system to restore normal cellular function. Understanding the structural and functional properties of HIP2 will not only deepen our comprehension of protein regulation but may also pave the way for innovative therapeutic approaches targeting diseases associated with dysregulated ubiquitination.
