Analytical Data
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基因名
nucB
- Application
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别名
nucB; BSU25750; Sporulation-specific extracellular nuclease; EC 3.-.-.-
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种属
Bacillus subtilis
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表达系统
Yeast
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P42983
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表达区间
29-136aa
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分子量
12 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NucB, a nuclease enzyme derived from Bacillus subtilis, has garnered significant attention in the field of molecular biology and biotechnology due to its unique characteristics and potential applications. As a member of the DUF1594 family of proteins, NucB exhibits endonuclease activity that can efficiently degrade RNA, making it a valuable tool for various research and therapeutic applications, such as antisense RNA delivery and nucleotide manipulation. The interest in NucB is further fueled by its structural stability and favorable reaction conditions, which help overcome challenges faced with other nucleases. Studies on the recombinant expression of NucB in heterologous systems, such as Escherichia coli, have enabled researchers to produce large quantities of this enzyme for in-depth characterization and functional analysis. Understanding the mechanisms underlying NucB's activity can lead to innovative uses in genetic engineering, RNA biology, and therapeutic interventions. Moreover, the insights gained from NucB research may contribute to the development of new biotechnological tools and strategies for RNA-based therapies, thereby enhancing our ability to manipulate RNA molecules for research and clinical purposes. This growing body of knowledge not only highlights the significance of NucB in scientific exploration, but also paves the way for novel applications in the emerging field of RNA therapeutics.
