Analytical Data
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Gene name
PCK2
- Application
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Alternative Names
PEPCK; PEPCK2
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16822
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Expression Region
Leu33~Val198
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Molecular Weight
24kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PCK2 recombinant protein research is primarily focused on understanding the role of phosphoenolpyruvate carboxykinase 2 (PCK2) in metabolic pathways, particularly in gluconeogenesis, which is the process of synthesizing glucose from non-carbohydrate precursors. PCK2 is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, a key step in this metabolic pathway. Its relevance has gained attention due to its potential implications in various diseases, including diabetes and cancer, where altered glucose metabolism is a hallmark. Furthermore, the regulation and expression of PCK2 can vary among different tissues, making it an intriguing target for therapeutic interventions. The recombinant production of PCK2 facilitates in-depth studies and characterizations of its enzymatic activity, structural properties, and interaction with other metabolic pathways. Understanding the mechanisms by which PCK2 influences glucose homeostasis and cellular energy balance can provide valuable insights for developing novel strategies aimed at treating metabolic disorders. As a result, PCK2 recombinant protein research is a vital field that integrates biochemistry, molecular biology, and medical science, highlighting its significance in both basic research and clinical applications.
