Analytical Data
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Gene name
ribA
- Application
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Alternative Names
GTP cyclohydrolase II
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A7I7
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Expression Region
1-196aa
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Molecular Weight
25.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RibA (riboflavin biosynthesis protein A) is a crucial enzyme involved in the riboflavin (vitamin B2) biosynthetic pathway, primarily found in bacteria. Understanding RibA is significant due to the essential role riboflavin plays in cellular metabolism and energy production. Its importance extends to medical and agricultural fields, where it can influence microbial growth and development. Recent studies have highlighted that the riboflavin biosynthetic pathway represents an attractive target for antibiotic development, especially in pathogenic bacteria that rely on this pathway for survival. The characterization of RibA, particularly through recombinant protein technology, enables researchers to explore its structure, function, and potential as a target for drug design. Advances in genetic engineering allow for the production of recombinant RibA, facilitating detailed biochemical studies and high-throughput screening for inhibitors. Additionally, understanding RibA's regulatory mechanisms can provide insights into metabolic pathways, enhancing our knowledge of bacterial physiology and aiding in the development of novel therapeutic strategies. The investigation of RibA and its interactions within the riboflavin synthesis pathway could pave the way for innovative approaches in tackling antibiotic resistance and improving agricultural practices by managing beneficial and pathogenic bacterial populations. Overall, the research surrounding RibA encompasses aspects of microbiology, biochemistry, and pharmacology, making it a focal point for interdisciplinary studies aimed at addressing contemporary challenges in health and food security.
