Analytical Data
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Gene name
MCP-3/CCL7
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简介
MCP-3/CCL7 Protein, Mouse is a CC chemokine and elicitor that binds to CCR1, CCR2 and CCR3 to mediate antiviral, antibacterial, antitumor and other immune responses. MCP-3/CCL7 Protein, Mouse is a recombinant mouse MCP-3/CCL7 protein expressed by E.coilMCP-3/CCL7(Q24-P97)[1].
- Application
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Biological Activity
1.Full biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml. 2.Measured by its ability to chemoattract THP-1 human monocytes. The ED50 this effect is 153.9 ng/mL, corresponding to a specific activity is 6497.726 U/mg. 3.Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A.The ED50 for this effect is 0.7142 μg/mL, corresponding to a specific activity is 1400.168 U/mg. Measured by its ability to chemoattract THP-1 human monocytes. The ED50 for this effect is 153.9 ng/mL, corresponding to a specific activity is 6497.726 U/mg.
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Alternative Names
rMuMCP-3/CCL7; C-C motif chemokine 7; MCP3; SCYA7
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
Q03366
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Expression Region
Q24-P97
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Protein Length
Full Length of Mature Protein
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Molecular Weight
12 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MCP-3 (Monocyte Chemoattractant Protein-3), also known as CCL7 (Chemokine C-C motif ligand 7), is a member of the chemokine family, which plays a crucial role in the recruitment and activation of immune cells during inflammatory responses. Research on MCP-3/CCL7 has gained attention due to its involvement in various pathological conditions, including autoimmune diseases, cancer, and obesity-related inflammation. This chemokine is primarily produced by activated macrophages and other immune cells, facilitating the migration of monocytes, T cells, and basophils to sites of inflammation. Understanding the mechanisms of MCP-3/CCL7 function is essential for exploring novel therapeutic targets for diseases characterized by chronic inflammation. The recombinant protein derived from MCP-3/CCL7 allows for detailed studies of its biological activities and interaction with specific receptors, contributing to insights into its role in disease processes and potential applications in immunotherapy. Moreover, the ability to produce this chemokine in a recombinant form enables researchers to investigate its structure-function relationships and the development of antagonists or mimetics that may aid in the modulation of immune responses. Overall, the study of MCP-3/CCL7 recombinant protein represents a significant avenue in immunology and therapeutic research, enhancing our understanding of immune regulation and offering potential strategies for intervention in various inflammatory diseases.
