Analytical Data
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Gene name
ALOXE3
- Application
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Alternative Names
ALOX-E3; LOX3; eLOX3; E-LOX; Epidermis-Type Lipoxygenase 3; Hydroperoxy icosatetraenoate dehydratase; Hydroperoxide isomerase ALOXE3
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BYJ1
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Expression Region
Met1~Ile711
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Molecular Weight
84kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ALOXE3 (Arachidonate Lipoxygenase 3) is an enzyme that plays a critical role in the metabolism of polyunsaturated fatty acids, particularly in the synthesis of specialized pro-resolving mediators. This enzyme is predominantly expressed in the skin and is essential for maintaining epidermal barrier function, regulating inflammation, and mediating allergic responses. Mutations in the ALOXE3 gene are linked to various skin disorders, including a rare genetic condition known as congenital ichthyosiform erythroderma, characterized by severe skin dryness and scaling. Given its pivotal role in skin physiology and pathology, the research on recombinant ALOXE3 protein has gained attention. Understanding its structure, enzymatic activity, and interactions with other cellular components can provide insights into its function and potential therapeutic targets. Moreover, recombinant ALOXE3 protein may serve as a valuable tool for developing novel treatments for skin-related diseases and enhancing our understanding of lipid mediators in inflammatory responses. Therefore, the study of ALOXE3 not only expands our comprehension of skin biology but also holds promise for innovative approaches in dermatology and anti-inflammatory therapies.
