Analytical Data
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Gene name
PUF60
- Application
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Alternative Names
FIR; SIAHBP1; RoBPI; Fuse Binding Protein Interacting Repressor; Siah Binding Protein 1; Pyrimidine Tract Binding Splicing Factor; Ro Ribonucleoprotein Binding Protein 1
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Species
Human
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Source
E. coli
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Tag
N- His & GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UHX1
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Expression Region
Asn226~Gln549
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Molecular Weight
65kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PUF60 (Poly(U)-binding Splicing Factor 60) is a multifunctional protein that plays a crucial role in regulating alternative splicing, RNA stability, and gene expression in eukaryotic cells. It belongs to the PUF (Pumilio and FBF) family of RNA-binding proteins, which are characterized by their ability to recognize and bind to specific RNA sequences, thereby modulating the post-transcriptional fate of their target mRNAs. Research into PUF60 has gained traction due to its involvement in essential biological processes, including embryonic development, cell differentiation, and response to stress. Dysregulation of PUF60 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. Studies have shown that PUF60 interacts with several key splicing factors and components of the RNA processing machinery, suggesting a complex network of regulatory interactions. The recombinant expression of PUF60 allows for detailed biochemical and biophysical characterization, enabling researchers to explore its RNA-binding characteristics, structural properties, and functional dynamics in splicing regulation. Understanding the molecular mechanisms by which PUF60 influences gene expression may provide insights into its role in normal cellular function and disease pathology, making it an important focus for future research endeavors in molecular biology and therapeutic developments.
