Analytical Data
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基因名
R-Cadherin/CDH4
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简介
The R-Cadherin/CDH4 protein facilitates cell adhesion through homophilic interactions, aiding in cell connections and potential cell type sorting. It may play a crucial role in retinal development, indicating its significance in orchestrating cellular processes within the context of eye development and function. R-Cadherin/CDH4 Protein, Human (HEK293, His) is the recombinant human-derived R-Cadherin/CDH4 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 0.3577 μg/mL, corresponding to a specific activity is 2.796×104 units/mg. Measured in a cell proliferation assay using SH-SY5Y cells. The ED50 for this effect is 0.3577 μg/mL , corresponding to a specific activity is 2.796×104 units/mg.
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别名
Cadherin-4; Retinal cadherin; R-CAD; R-cadherin
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P55283
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表达区间
H21-A734
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氨基酸序列
HNEDLTTRETCKAGFSEDDYTALISQNILEGEKLLQVKFSSCVGTKGTQYETNSMDFKVGADGTVFATRELQVPSEQVAFTVTAWDSQTAEKWDAVVRLLVAQTSSPHSGHKPQKGKKVVALDPSPPPKDTLLPWPQHQNANGLRRRKRDWVIPPINVPENSRGPFPQQLVRIRSDKDNDIPIRYSITGVGADQPPMEVFSIDSMSGRMYVTRPMDREEHASYHLRAHAVDMNGNKVENPIDLYIYVIDMNDNRPEFINQVYNGSVDEGSKPGTYVMTVTANDADDSTTANGMVRYRIVTQTPQSPSQNMFTINSETGDIVTVAAGLDREKVQQYTVIVQATDMEGNLNYGLSNTATAIITVTDVNDNPPEFTASTFAGEVPENRVETVVANLTVMDRDQPHSPNWNAVYRIISGDPSGHFSVRTDPVTNEGMVTVVKAVDYELNRAFMLTVMVSNQAPLASGIQMSFQSTAGVTISIMDINEAPYFPSNHKLIRLEEGVPPGTVLTTFSAVDPDRFMQQAVRYSKLSDPASWLHINATNGQITTAAVLDRESLYTKNNVYEATFLAADNGIPPASGTGTLQIYLIDINDNAPELLPKEAQICEKPNLNAINITAADADVDPNIGPYVFELPFVPAAVRKNWTITRLNGDYAQLSLRILYLEAGMYDVPIIVTDSGNPPLSNTSIIKVKVCPCDDNGDCTTIGAVAAAGLGTGA
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蛋白长度
Partial
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分子量
90-115 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
R-Cadherin, also known as CDH4, is a member of the cadherin family of proteins, which are critical for cellular adhesion, tissue formation, and communication in various biological processes. R-Cadherin plays a significant role in the development of the nervous system and is considered essential for the maintenance of neuronal connections. Its expression is particularly prominent in the retina and other regions of the central nervous system, where it is involved in synapse formation and stability. Given its vital functions, the study of R-Cadherin/CDH4 has garnered attention in both developmental biology and neurobiology, as alterations in cadherin function can lead to neurological disorders and cancer progression. Researchers aim to characterize the structure and functional properties of recombinant R-Cadherin to better understand its role in cell adhesion and signaling pathways. This involves producing R-Cadherin as a recombinant protein to facilitate in vitro studies, enabling detailed analysis of its interactions with other cellular components as well as its potential implications in therapeutic applications. By exploring the mechanistic pathways through which R-Cadherin influences cellular behaviors, researchers hope to uncover insights that could inform strategies for addressing diseases associated with cadherin dysfunction, thereby paving the way for novel treatments in regenerative medicine and oncology.
