Analytical Data
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Gene name
ARHGEF18
- Application
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Alternative Names
114KDA Rho-specific guanine nucleotide exchange factor ;p114-Rho-GEF ;p114RhoGEFSeptin-associated RhoGEF ;SA-RhoGEF
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6ZSZ5
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Expression Region
159-551aa
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Molecular Weight
71.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ARHGEF18 (Rho guanine nucleotide exchange factor 18) is a critical protein that functions as a regulator of the Rho family of GTPases, which are integral to various cellular processes including cytoskeletal dynamics, cell migration, and signal transduction. Dysregulation of RhoGTPases has been implicated in several pathological conditions, including cancer, making ARHGEF18 a potential target for therapeutic intervention. The protein interacts with different signaling pathways, influencing cellular responses to external stimuli and thereby playing a pivotal role in maintaining cellular homeostasis. Recent studies have highlighted the importance of ARHGEF18 in modulating immune responses and its involvement in neural development. Understanding the structure and function of ARHGEF18 through recombinant protein studies could provide insights into its role in health and disease. Furthermore, the expression and purification of ARHGEF18 as a recombinant protein enable detailed biochemical and biophysical characterizations, facilitating the exploration of its interactions with RhoGTPases and other signaling molecules. This research is crucial for elucidating the mechanisms by which ARHGEF18 contributes to cellular signaling networks and its potential implications in targeted therapies for diseases associated with RhoGTPase dysregulation.
