Analytical Data
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Gene name
UQCRFS1
- Application
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Alternative Names
Complex III subunit 5Cytochrome b-c1 complex subunit 5Rieske iron-sulfur protein ;RISPUbiquinol-cytochrome c reductase iron-sulfur subunit
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P47985
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Expression Region
79-274aa
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Molecular Weight
48.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UQCRFS1 (Ubiquinone-Cytochrome c Reductase Iron-Sulfur Protein 1) is a crucial component of the mitochondrial electron transport chain, playing a significant role in cellular respiration and ATP production. It is part of the larger complex III, which is essential for oxidative phosphorylation, a process whereby energy stored in nutrients is converted into ATP with the help of oxygen. Deficiencies or mutations in UQCRFS1 have been linked to various mitochondrial disorders, resulting in compromised energy metabolism and contributing to conditions like neurodegenerative diseases and muscle weakness. Recent studies have focused on understanding its structure-function relationships, the mechanisms of its redox chemistry, and its role in mitochondrial bioenergetics. Additionally, UQCRFS1 is being explored as a potential target for therapeutic interventions aimed at restoring mitochondrial function in affected individuals. Through the generation of recombinant UQCRFS1 proteins, researchers aim to dissect its molecular mechanisms, study its interactions with other mitochondrial components, and establish its role in disease pathology. The insights gained from these studies could pave the way for novel treatments for mitochondrial diseases and enhance our understanding of cellular energy regulation.
