Analytical Data
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基因名
TCN2/Transcobalamin-2
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简介
The TCN2/Transcobalamin-2 protein plays a key role as the primary vitamin B12 binding and transporter, ensuring efficient delivery of cobalamin to cells.Its complex interactions involve binding to CD320, specifically to the LDL receptor class A domain.TCN2/Transcobalamin-2 Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived TCN2/Transcobalamin-2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. Immobilized CD320 at 25 ng/mL (100 μL/well) can bind Biotinylated TCN2. The ED50 for this effect is 11.18 ng/mL, corresponding to a specific activity is 8.94×10^4 Unit/mg. Measured by its binding ability in a functional ELISA. Immobilized CD320 at 25 ng/mL (100 μL/well) can bind Biotinylated TCN2. The ED50 for this effect is 11.18 ng/mL, corresponding to a specific activity is 8.94×104 Unit/mg.
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别名
Transcobalamin-2; TC-2; TCII; Tcn2
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种属
Mouse
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O88968
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表达区间
E19-W430
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氨基酸序列
EFCVIPRIDSQLVEKLGQRLLPWMDRLSSEQLNPSVFVGLRLSSMQAGTKEDLYLHSLKIHYQQCLLRSTSSDDNSSCQPKLSGGSLALYLLALRANCEFFGSRKGDRLISQLKWFLEDEKKAIGHNHEGHPNTNYYQYGLSILALCVHQKRLHDSVVGKLLYAVEHDYFTYQGHVSVDTEAMAGLALTCLERFNFNSDLRPRITMAIETVREKILKSQAPEGYFGNIYSTPLALQMLMTSPASGVGLGTACIKAGTSLLLSLQDGAFQNPLMISQLLPILNHKTYLDLIFPDCQASRVMLVPAVEDPVHISEVISVTLKVASALSPYEQTFFVFAGSSLEDVLKLAQDGGGFTYGTQASLSGPYLTSVLGKDAGDREYWQLLRAPDTPLLQGIADYKPQDGETIELRLVRW
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蛋白长度
Full Length of Mature Protein
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分子量
42.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TCN2, or Transcobalamin-2, is a key protein responsible for the cellular uptake and transport of vitamin B12 (cobalamin). It plays a vital role in maintaining cobalamin homeostasis and facilitating its biosynthetic processes. Mutations and polymorphisms in the TCN2 gene have been associated with various clinical conditions, including neurological disorders, cardiovascular diseases, and certain types of anemia, highlighting its significance in human health. Given the crucial role of TCN2 in vitamin B12 metabolism, research into its recombinant protein form has gained traction. Recombinant TCN2 can be utilized in studying the mechanisms of cobalamin transport and signaling in different cellular contexts. Additionally, it serves as a potential candidate for developing therapeutic strategies aimed at addressing vitamin B12 deficiency and associated disorders. By producing and analyzing recombinant TCN2, researchers aim to elucidate its structure-function relationships, investigate its interactions with cellular receptors, and explore its role in cobalamin-mediated pathways. The findings from such studies are essential not only for understanding basic biological processes but also for paving the way for new approaches in clinical nutrition and therapeutic intervention.
