Analytical Data
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Gene name
GSTa5
- Application
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Alternative Names
GST-a5; Glutathione S-Transferase A5; GST class-alpha member 5
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Species
Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46418
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Expression Region
Met1~Ser221
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Molecular Weight
26kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GSTa5 recombinant protein has garnered significant attention in the field of biotechnology and molecular biology due to its versatile applications in protein purification, enzyme assays, and studies of protein interactions. Glutathione S-transferases (GSTs) are a superfamily of enzymes that play a crucial role in detoxifying harmful compounds by catalyzing the conjugation of glutathione to various substrates. The GSTa5 isoform, specifically, is known for its ability to bind to a wide range of hydrophobic compounds, making it an ideal candidate for the development of fusion proteins in research and therapeutic applications. The use of GSTa5 as a fusion partner allows for the simplification of protein purification processes through affinity chromatography, facilitating the isolation of target proteins from complex biological mixtures. Furthermore, research has indicated that GSTa5 may have implications in drug resistance, cancer biology, and the metabolism of xenobiotics, thereby expanding its relevance beyond mere protein purification. As scientists continue to explore the structural and functional characteristics of GSTa5, advances in recombinant DNA technologies have made it possible to produce this protein in high yields, enabling extensive biochemical studies. This research contributes to a better understanding of GSTa5’s role in cellular processes and its potential therapeutic applications, highlighting its significance in both fundamental and applied sciences.
