Analytical Data
-
基因名
IL-6
-
简介
IL-6 Protein is a multifunctional cytokine secreted by both immune and non-immune cells, and belongs to the interleukin family. IL-6 Protein plays a key role in immune responses, inflammatory reactions and other processes. IL-6 Protein, Mouse is a recombinant IL-6 protein expressed by E. coli without a tag[1][2][3].
- Application
-
生物活性
1.The ED50 is <0>5 × 107 units/mg. 2. Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-6 Protein is immobilized at 5 μg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse IL-6 R alpha (HY-P72538). The ED50 for this effect is 20-156 ng/mL. Immobilized Mouse IL-6, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha that produces 50% of the optimal binding response is found to be approximately 117.5 ng/mL.
-
别名
rMuIL-6; BSF-2; CDF; Hybridoma growth factor; IFN-beta-2
-
种属
Mouse
-
表达系统
E. coli
-
标签
Tag Free
-
纯度
Greater than 95% as determined by reducing SDS-PAGE.
-
蛋白编号
P08505
-
表达区间
F25-T211
-
氨基酸序列
FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT
-
蛋白长度
Full Length of Mature Protein
-
分子量
22 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a critical role in immune response, inflammation, and hematopoiesis. It is produced by various cell types, including T cells, macrophages, and fibroblasts, and its dysregulation is associated with numerous pathological conditions, such as autoimmune diseases, cancers, and chronic inflammatory disorders. Research into IL-6 has gained prominence due to its dual role as both a pro-inflammatory and anti-inflammatory mediator, which complicates its therapeutic targeting. Recombinant IL-6 proteins are essential tools for studying its biological effects and mechanisms of action. These proteins can be produced using recombinant DNA technology, allowing for the generation of large quantities of biologically active IL-6 for research purposes. Investigations involving IL-6 recombinant proteins have provided insights into signaling pathways, interactions with IL-6 receptors, and the cytokine’s role in disease processes. Furthermore, IL-6 has emerged as a potential therapeutic target, prompting the development of IL-6 inhibitors for treating various diseases, including rheumatoid arthritis and COVID-19. Understanding the structure-function relationship of IL-6 through recombinant protein studies is crucial for the design of effective interventions, making the research into IL-6 recombinant proteins a significant area in cellular and molecular biology, as well as in clinical applications.
