Analytical Data
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Gene name
RAD18
- Application
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Alternative Names
Postreplication repair protein RAD18 ;hHR18 ;hRAD18RING finger protein 73
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NS91
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Expression Region
1-495aa
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Molecular Weight
72.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RAD18 is a vital protein involved in the DNA damage response and the maintenance of genomic stability. It plays a critical role in the regulation of DNA repair pathways, particularly in the context of post-replication repair, where it facilitates the recruitment of other repair proteins to sites of DNA lesions. The study of RAD18 has gained prominence due to its implications in various biological processes including cell cycle progression, DNA damage signaling, and the accuracy of DNA replication. Abnormalities in RAD18 function have been linked to increased susceptibility to cancer, as defective DNA repair mechanisms can lead to the accumulation of mutations. Moreover, the protein has emerged as a potential target for cancer therapies, as exploiting its role in DNA repair may enhance the efficacy of chemotherapeutic agents. Recent studies have focused on elucidating the molecular mechanisms by which RAD18 operates, including its interactions with ubiquitin ligases and other key factors within the DNA repair machinery. Understanding RAD18's function not only provides insight into fundamental cellular processes but also opens avenues for the development of novel therapeutic strategies to combat cancer by targeting DNA repair pathways.
