Analytical Data
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基因名
GCLM
- Application
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别名
GLCLR; GECS; GCL; Gamma Glutamylcysteine Synthetase; Glutamate Cysteine Ligase Regulatory; Gamma-ECS regulatory subunit; Glutamate cysteine ligase modifier subunit
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48507
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表达区间
Ser40~Ser251
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分子量
28kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GCLM (glutamate-cysteine ligase modifier subunit) is an essential component of the γ-glutamylcysteine synthetase enzyme complex, which plays a critical role in the synthesis of glutathione, a key antioxidant in cellular defense against oxidative stress. The regulation of glutathione levels is crucial for maintaining cellular homeostasis and protecting cells from damage caused by reactive oxygen species (ROS). Dysregulation of GCLM has been implicated in various pathological conditions, including neurodegenerative diseases, cancer, and metabolic disorders. Understanding the structure and function of GCLM is therefore vital for elucidating its role in disease processes and for developing therapeutic strategies aimed at modulating glutathione levels. Recombinant GCLM proteins have been produced for enzymatic assays, structural studies, and potential therapeutic applications. By investigating the biochemical properties of GCLM and its interaction with other cellular components, researchers aim to uncover novel insights into cellular oxidative stress responses and to identify potential targets for drug development. The ongoing research on GCLM reaffirms its significance in both basic and applied biomedical sciences, making it a focal point for understanding disease mechanisms and exploring innovative treatment approaches.
