Analytical Data
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基因名
MIS12
- Application
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别名
MIS12; Protein MIS12 homolog
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种属
Human
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H081
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表达区间
1-205aa
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分子量
40.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MIS12, a crucial protein in the spindle assembly checkpoint, plays a vital role in ensuring accurate chromosome segregation during cell division. It is part of a larger protein complex that connects kinetochores to microtubules, facilitating the proper attachment and alignment of chromosomes on the mitotic spindle. Research has indicated that misregulation of MIS12 can lead to chromosomal instability and has been implicated in various cancers and developmental disorders. The study of MIS12 reconstituted proteins is particularly significant as it allows scientists to delineate the molecular mechanisms of kinetochore function and its interaction with microtubules. By reconstituting MIS12 in vitro, researchers can investigate its structural properties, binding affinities, and the dynamics of its interactions within the kinetochore complex. This research serves not only to enhance our understanding of mitotic processes but also to identify potential therapeutic targets for diseases associated with chromosomal aberrations. Overall, the investigation of MIS12 and its reconstituted proteins provides insight into fundamental cellular processes that are critical for maintaining genomic stability.
