Analytical Data
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Gene name
IRF3
- Application
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Alternative Names
IIAE7; Interferon regulatory factor 3; IRF 3; IRF-3; IRF3; IRF3_HUMAN; MGC94729
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14653
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Expression Region
M1-S427
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AA Sequence
MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHPLSLTSDQYKAYLQDLVEGMDFQGPGES
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Protein Length
Full Length
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Molecular Weight
59 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
IRF3 (Interferon Regulatory Factor 3) is a crucial transcription factor that plays a significant role in the innate immune response, particularly in the regulation of type I interferon (IFN) production following viral infections. It is activated by various pattern recognition receptors, leading to its phosphorylation and subsequent dimerization, which translocates to the nucleus to initiate the transcription of IFN genes and other immune-related factors. Research on recombinant IRF3 protein is essential for understanding its structural and functional properties, interactions with other signaling molecules, and its overall role in antiviral responses. Scientists have focused on elucidating the mechanisms by which IRF3 is activated and how it mediates downstream signaling pathways linked to immune modulation. Producing and studying recombinant IRF3 protein allows researchers to investigate its oligomerization, post-translational modifications, and interaction with other proteins, paving the way for novel therapeutic strategies to enhance antiviral defenses or modulate inflammatory responses in various diseases. Additionally, the manipulation of IRF3 activity may provide insights into its potential role in cancer immunity and other disorders characterized by dysregulated immune responses. Thus, the exploration of IRF3 through recombinant protein technology not only expands our knowledge of the immune system but also opens avenues for the development of targeted therapies that harness the power of innate immunity.
