Analytical Data
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Gene name
CD45
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简介
Receptor-type tyrosine-protein phosphatase C (PTPRC) is a receptor type PTP and an essential positive regulator of T- and B-cell antigen receptor signaling. PTPRC functions through direct interaction with antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. PTPRC also suppresses JAK kinases as a regulator of cytokine receptor signaling. CD45 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD45 protein, expressed by HEK293 , with C-His, C-Avi labeled tag.
- Application
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Alternative Names
L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08575-4
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Expression Region
Q26-K416
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AA Sequence
QSPTPSPTDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK
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Protein Length
Partial
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Molecular Weight
80-110 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CD45, also known as leukocyte common antigen, is a crucial glycoprotein expressed on the surface of all nucleated hematopoietic cells. It plays a significant role in regulating T and B cell signaling, activation, and development, making it an essential component in the immune response. The study of CD45 has gained momentum due to its involvement in various diseases, including autoimmune disorders, cancers, and infectious diseases. Understanding the structure and function of CD45, especially its isoforms resulting from alternative splicing, is vital for elucidating its complex role in immune regulation. Recent advances in recombinant protein technology have enabled the production of CD45 isoforms, allowing researchers to investigate their specific biological functions and therapeutic potentials. Additionally, recombinant CD45 proteins are being explored for use in diagnostic assays and as potential targets for immunotherapy. Overall, the ongoing research on CD45 recombinant proteins holds promise for developing novel strategies to manipulate the immune system for better disease management.
