Analytical Data
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Gene name
H2B
- Application
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Species
Human, Mouse, Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
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Expression Region
Pro2~Lys126
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Molecular Weight
18kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
H2B recombinant proteins have garnered significant attention in the field of molecular biology and biochemistry due to their pivotal role in chromatin structure and gene expression regulation. Histone H2B is one of the core components of the nucleosome, around which DNA coils, thus influencing DNA accessibility for transcription, replication, and repair processes. The study of H2B, particularly in its modified forms, has revealed critical insights into epigenetic mechanisms, such as histone acetylation and methylation, which can alter gene expression without changing the underlying DNA sequence. The recombinant production of H2B allows researchers to obtain pure, homogeneous proteins for detailed structural and functional studies. Moreover, understanding the dynamics of H2B interactions with other histones and regulatory proteins can shed light on the complex processes of chromatin remodeling and transcriptional regulation. This research has implications for various diseases, including cancer, where aberrant histone modifications are often observed. Consequently, H2B recombinant protein studies contribute to the broader understanding of cellular mechanisms and hold potential for therapeutic advancements in epigenetic therapies.
