Analytical Data
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Gene name
Sox2
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简介
Sox2 protein is a key transcription factor that forms a trimeric complex with OCT4 and controls the expression of genes critical for embryonic development (YES1, FGF4, UTF1, ZFP206). It binds the NANOG enhancer and is critical for early embryogenesis and stem cell pluripotency. Sox2 Protein, Human is the recombinant human-derived Sox2 protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
SOX2; Transcription factor SOX-2
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48431
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Expression Region
R40-D123
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Protein Length
Partial
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Molecular Weight
10.4 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sox2, a member of the SRY-related HMG-box (Sox) transcription factor family, plays a crucial role in maintaining stem cell pluripotency and self-renewal, making it a significant focus in stem cell biology and regenerative medicine. Its expression is primarily observed in embryonic stem cells and certain adult stem cells, suggesting a pivotal function in early development and tissue homeostasis. Research has shown that Sox2 collaborates with other transcription factors, such as Oct4 and Nanog, to form a core regulatory network essential for maintaining the undifferentiated state of stem cells. Moreover, Sox2's involvement in cellular reprogramming has made it a key component in generating induced pluripotent stem cells (iPSCs) from somatic cells, which has implications for both disease modeling and therapeutic applications. The study of recombinant Sox2 protein is critical for deciphering its mechanisms of action and interactions at the molecular level. By producing and characterizing Sox2 as a recombinant protein, researchers can investigate its structural properties, binding affinities, and functional interactions with other proteins and DNA. This knowledge is vital for understanding the role of Sox2 in stem cell biology and its potential manipulation in regenerative therapies. Furthermore, exploring Sox2's role in various pathological conditions, such as cancer, offers insights into its potential as a therapeutic target or biomarker. Consequently, the investigation of recombinant Sox2 protein forms the cornerstone of fundamental and applied research aimed at harnessing the power of stem cells for innovative medical solutions.
