Analytical Data
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Gene name
Klotho beta
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简介
Klotho beta protein represses cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. It enhances binding between fibroblast growth factor receptors and fibroblast growth factor 21, and directly interacts with FGF19 and FGF21. This positions Klotho beta as a crucial modulator of metabolic and growth factor signaling pathways. Klotho beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived Klotho beta protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Biological Activity
Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED50 for this effect is 291.3 ng/mL. Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED50 for this effect is 291.3 ng/mL.
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Alternative Names
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; klotho beta-like protein
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Species
Mouse
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q99N32
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Expression Region
F53-L995
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Protein Length
Partial
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Molecular Weight
125-130 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Klotho beta (KLB) is a protein that has garnered significant attention in recent years due to its potential roles in aging, metabolism, and various disease processes. First identified as a member of the Klotho family of proteins, KLB is thought to function as a co-receptor for fibroblast growth factor 21 (FGF21), a key regulator of energy metabolism. Research indicates that KLB can influence pathways related to insulin sensitivity, lipid metabolism, and glucose homeostasis, making it of particular interest in the context of metabolic disorders such as obesity and diabetes. Additionally, studies have suggested that KLB may play a role in age-related decline in physiological functions, with low levels of KLB being associated with senescence and various age-related diseases. As a result, the recombinant expression and purification of Klotho beta protein have become a focal point for researchers aiming to elucidate its biological functions and therapeutic potential. Investigating KLB in vitro and in vivo could pave the way for novel treatments targeting metabolic diseases and aging processes, thus enhancing our understanding of healthspan and lifespan regulation. Given its dual role in metabolism and aging, KLB represents a promising area for future research, particularly in the development of targeted therapies that harness its beneficial effects on health.
