Analytical Data
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Gene name
LIV-1/SLC39A6
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简介
The LIV-1/SLC39A6 protein is an important zinc influx transporter that maintains zinc balance and drives epithelial-to-mesenchymal transition (EMT). It cooperates with SLC39A10 to trigger EMT by promoting zinc uptake, regulating NCAM1 phosphorylation, and integrating it into focal adhesion complexes. LIV-1/SLC39A6 Protein, Rat (sf9, His) is the recombinant rat-derived LIV-1/SLC39A6 protein, expressed by sf9 , with C-His labeled tag.
- Application
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Alternative Names
SLC39A6; LIV-1; ZIP6; Zinc transporter ZIP6; ZIP-6
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Species
Rat
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q4V887
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Expression Region
L21-W311
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Protein Length
Extracellular Domain
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Molecular Weight
46 kDa. The reducing (R) protein migrat es as 46 kDa in SDS-PAGE may be due to glycosylation.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LIV-1, also known as SLC39A6, is a member of the ZIP (Zinc-regulated transporter, Iron-regulated transporter-like protein) family, playing a crucial role in zinc homeostasis within cells. Research into LIV-1 has gained prominence due to its implications in cancer biology, particularly in breast cancer, where it has been identified as a potential biomarker and therapeutic target. High expression levels of LIV-1 are associated with tumor progression, metastasis, and poor patient prognosis. Moreover, LIV-1 is implicated in the transportation of zinc and other metal ions, which are vital for various cellular processes, including enzyme activity, gene expression, and cell signaling. Given the essential role of zinc in regulating cellular functions, understanding the structure and function of LIV-1/SLC39A6 is critical for elucidating its involvement in carcinogenesis and other disease states. Recent studies have focused on the recombinant production of LIV-1 protein to facilitate structure-function analyses and high-throughput screening for potential inhibitors. By characterizing this protein, researchers aim to uncover its mechanism of action in metal ion transport and its contribution to tumor behavior, ultimately paving the way for the development of novel therapeutic strategies targeting LIV-1 in cancer treatment.
