Analytical Data
-
基因名
Cytochrome b5/CYB5A
-
简介
Cytochrome b5 (CYB5A) is a vital membrane-bound hemoprotein acting as an electron carrier for diverse membrane-bound oxygenases. Positioned in cellular membranes, it crucially facilitates electron transfer processes, supporting the activity of oxygenase enzymes. CYB5A's role as an electron carrier highlights its significance in cellular redox reactions and metabolic pathways involving oxygenases. Cytochrome b5/CYB5A Protein, Human (His) is the recombinant human-derived Cytochrome b5/CYB5A protein, expressed by E. coli , with N-6*His labeled tag.
- Application
-
别名
rHuCytochrome b5/CYB5A, His; Cytochrome b5; Microsomal Cytochrome b5 Type A; MCB5; CYB5A; CYB5
-
种属
Human
-
表达系统
E. coli
-
标签
N-6*His
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P00167
-
表达区间
M1-D134
-
蛋白长度
Full Length
-
分子量
17 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Cytochrome b5 (CYB5A) is a heme-containing electron transport protein that plays a crucial role in various biological processes, such as fatty acid desaturation, cholesterol synthesis, and the metabolism of drugs and toxins. Its significance arises from its involvement in the electron transfer reactions that facilitate the functioning of several enzymatic pathways, particularly those linked to the endoplasmic reticulum. The precise functioning of CYB5A is pivotal for maintaining cellular homeostasis and regulating lipid metabolism, which has implications for conditions such as obesity and metabolic disorders. Research into recombinant CYB5A has gained traction due to its potential applications in biotechnology and medicine, including its role as a probe for studying electron transfer mechanisms and its utility in enhancing the activity of membrane-bound enzymes. By employing recombinant DNA technology, researchers are able to produce CYB5A in higher quantities and purities, facilitating detailed structural and functional studies. Understanding CYB5A's interactions at the molecular level can unveil insights into its physiological roles and may lead to therapeutic innovations, particularly in areas related to metabolic diseases and pharmacology. Thus, the exploration of recombinant CYB5A not only enhances our fundamental understanding of biological processes but also opens avenues for biotechnological advancements and clinical applications.
