Analytical Data
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Gene name
Cytochrome b5/CYB5A
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简介
Cytochrome b5 (CYB5A) is a vital membrane-bound hemoprotein acting as an electron carrier for diverse membrane-bound oxygenases. Positioned in cellular membranes, it crucially facilitates electron transfer processes, supporting the activity of oxygenase enzymes. CYB5A's role as an electron carrier highlights its significance in cellular redox reactions and metabolic pathways involving oxygenases. Cytochrome b5/CYB5A Protein, Human (His) is the recombinant human-derived Cytochrome b5/CYB5A protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
rHuCytochrome b5/CYB5A, His; Cytochrome b5; Microsomal Cytochrome b5 Type A; MCB5; CYB5A; CYB5
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P00167
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Expression Region
M1-D134
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Protein Length
Full Length
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Molecular Weight
17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cytochrome b5 (CYB5A) is a heme-containing electron transport protein that plays a crucial role in various biological processes, such as fatty acid desaturation, cholesterol synthesis, and the metabolism of drugs and toxins. Its significance arises from its involvement in the electron transfer reactions that facilitate the functioning of several enzymatic pathways, particularly those linked to the endoplasmic reticulum. The precise functioning of CYB5A is pivotal for maintaining cellular homeostasis and regulating lipid metabolism, which has implications for conditions such as obesity and metabolic disorders. Research into recombinant CYB5A has gained traction due to its potential applications in biotechnology and medicine, including its role as a probe for studying electron transfer mechanisms and its utility in enhancing the activity of membrane-bound enzymes. By employing recombinant DNA technology, researchers are able to produce CYB5A in higher quantities and purities, facilitating detailed structural and functional studies. Understanding CYB5A's interactions at the molecular level can unveil insights into its physiological roles and may lead to therapeutic innovations, particularly in areas related to metabolic diseases and pharmacology. Thus, the exploration of recombinant CYB5A not only enhances our fundamental understanding of biological processes but also opens avenues for biotechnological advancements and clinical applications.
