Analytical Data
-
Gene name
MBP
-
简介
MBP proteins, especially the classical isoforms 4-13, dominate myelin membranes in the central nervous system and are critical for myelination and stability.In contrast, the non-canonical isoform 1-3/Golli-MBP may be involved in early brain development and contribute to transcriptional complexes that influence multiple cellular processes.MBP Protein, Mouse (His) is the recombinant mouse-derived MBP protein, expressed by E.coli , with N-His labeled tag.
- Application
-
Alternative Names
MBP; Hmbpr; MGC99675; mld; Myelin A1 ; myelin basic; shi
-
Species
Mouse
-
Source
E. coli
-
Tag
N-8*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P04370
-
Expression Region
M1-R250
-
Protein Length
Full Length
-
Molecular Weight
30-40 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MBP (Maltose-Binding Protein) recombinant proteins have garnered significant attention in the fields of molecular biology and biotechnology due to their unique properties and utility as versatile fusion tags. Initially characterized for their role in bacterial maltose transport systems, MBP has been widely adopted in recombinant protein technology to enhance solubility and facilitate the purification of various target proteins. Given its ability to form stable, soluble complexes, MBP is particularly beneficial for expressing proteins that are typically prone to aggregation or misfolding, thereby improving the yield and functionality of these proteins. Studies have demonstrated that MBP can be effectively used to increase the solubility of difficult-to-express proteins, making it a valuable tool for structural and functional studies. Furthermore, the presence of specific cleavage sites allows for easy removal of the MBP tag post-purification, enabling researchers to obtain the native form of the target protein for downstream applications. The versatility of MBP also extends to applications in drug development, vaccine design, and biosensor technology. As research progresses, there is ongoing exploration into optimizing MBP fusion strategies to enhance its efficacy and expand its potential applications, establishing MBP as a cornerstone in the recombinant protein production landscape.
