Analytical Data
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基因名
Carbonic Anhydrase 14
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简介
The carbonic anhydrase 14 (CA14) protein catalyzes the reversible hydration of carbon dioxide, converting it into bicarbonate ions and protons.Its enzymatic activity is essential for physiological processes, regulating pH and maintaining acid-base balance.Carbonic Anhydrase 14 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Carbonic Anhydrase 14 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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别名
Carbonic Anhydrase 14; Carbonate Dehydratase XIV; Carbonic Anhydrase XIV; CA-XIV; CA14;
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种属
Mouse
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9WVT6
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表达区间
A16-M290
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氨基酸序列
ADGGHHWTYEGPHGQDHWPTSYPECGGDAQSPINIQTDSVIFDPDLPAVQPHGYDQLGTEPLDLHNNGHTVQLSLPPTLHLGGLPRKYTAAQLHLHWGQRGSLEGSEHQINSEATAAELHVVHYDSQSYSSLSEAAQKPQGLAVLGILIEVGETENPAYDHILSRLHEIRYKDQKTSVPPFSVRELFPQQLEQFFRYNGSLTTPPCYQSVLWTVFNRRAQISMGQLEKLQETLSSTEEDPSEPLVQNYRVPQPLNQRTIFASFIQAGPLYTTGEM
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蛋白长度
Extracellular Domain
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分子量
41-50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Carbonic Anhydrase 14 (CA14) is a member of the carbonic anhydrase family of enzymes, which catalyze the reversible hydration of carbon dioxide to bicarbonate and protons, playing a crucial role in various physiological processes, including respiration, acid-base balance, and electrolyte transport. Unlike other carbonic anhydrases, CA14 is primarily expressed in the brain and the gastrointestinal tract, suggesting distinct functions in these tissues. Research has shown that CA14 may be involved in neuroprotection, synaptic function, and the regulation of ion transport in neurons. Its unique expression pattern and potential physiological roles have drawn interest in understanding its structure-function relationship. Recombinant CA14 protein is pivotal for studying the enzyme's catalytic mechanisms, inhibitor binding, and its role in various diseases, including neurological disorders. By producing CA14 as a recombinant protein, researchers can investigate its enzymatic properties, assess its interactions with potential drug compounds, and explore its therapeutic prospects. Additionally, the study of CA14 can contribute to our understanding of not only carbonate chemistry in biological systems but also its implications in pathophysiological conditions, thereby expanding the scope of carbonic anhydrases in biomedical research.
