Analytical Data
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Gene name
IDS/Iduronate 2-sulfatase
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简介
IDS/Iduronate 2-sulfatase Protein, a lysosomal enzyme, participates in the degradation pathway of dermatan sulfate and heparan sulfate. IDS/Iduronate 2-sulfatase Protein, Human (HEK293, His) is the recombinant human-derived IDS/Iduronate 2-sulfatase protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
Iduronate 2-sulfatase; Alpha-L-iduronate sulfate sulfatase; IDS; SIDS
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P22304-1
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Expression Region
S26-P550
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AA Sequence
SETQANSTTDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQRKIRQSYFASVSYLDTQVGRLLSALDDLQLANSTIIAFTSDHGWALGEHGEWAKYSNFDVATHVPLIFYVPGRTASLPEAGEKLFPYLDPFDSASQLMEPGRQSMDLVELVSLFPTLAGLAGLQVPPRCPVPSFHVELCREGKNLLKHFRFRDLEEDPYLPGNPRELIAYSQYPRPSDIPQWNSDKPSLKDIKIMGYSIRTIDYRYTVWVGFNPDEFLANFSDIHAGELYFVDSDPLQDHNMYNDSQGGDLFQLLMP
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
75-95 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Iduronate 2-sulfatase (IDS) is a critical enzyme involved in the degradation of glycosaminoglycans (GAGs), specifically heparan sulfate and dermatan sulfate. Deficiencies or mutations in the IDS gene lead to mucopolysaccharidosis type II (MPS II), a rare X-linked genetic disorder characterized by the accumulation of GAGs in various tissues, resulting in progressive physical and neurological symptoms. Research on recombinant IDS protein has gained significant attention due to its potential therapeutic application in enzyme replacement therapy (ERT) for MPS II patients. By producing active recombinant IDS, scientists aim to provide a consistent and effective treatment option to replace the deficient enzyme. Advances in recombinant protein technology have enabled the optimization of IDS expression and purification processes, enhancing enzyme stability and activity. Furthermore, studies have focused on the enzyme's structure-function relationship, elucidating the effects of specific mutations on its activity. Continued research into IDS not only enhances our understanding of MPS II pathogenesis but also improves the development of innovative therapies for lysosomal storage disorders.
