Analytical Data
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Gene name
BLMH/Bleomycin Hydrolase
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简介
Bleomycin Hydrolase Protein (BLMH) is crucial for cellular defense, inactivating the antitumor drug Bleomycin (BLM) by hydrolyzing its carboxamide bond. This neutralizes BLM's cytotoxic effects, protecting normal and malignant cells. BLMH's exact physiological function is unknown, but its significance in maintaining cellular balance and defending against harmful agents is evident. BLMH/Bleomycin Hydrolase Protein, Mouse (His) is the recombinant mouse-derived BLMH/Bleomycin Hydrolase Protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Bleomycin hydrolase; BH; BLM hydrolase; BMH
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8R016
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Expression Region
N2-E455
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Protein Length
Partial
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Molecular Weight
47 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Bleomycin hydrolase (BLMH) is acritical enzyme that plays a significant role in the metabolism of bleomycin, an antitumor antibiotic commonly used in cancer therapy. The enzyme is responsible for the hydrolysis of the bleomycin core structure, which converts it into non-toxic metabolites, thereby modulating the drug's efficacy and toxicity. Consequently, understanding BLMH's function and regulation is crucial for optimizing bleomycin treatment regimens and minimizing side effects in patients. Research on recombinant BLMH has gained traction due to advancements in genetic engineering techniques, allowing for the production of large quantities of the enzyme for detailed biochemical studies. These studies aim to elucidate the enzymatic mechanism, substrate specificity, and potential therapeutic applications of BLMH in drug resistance scenarios. Furthermore, recombinant BLMH can serve as a valuable tool in investigating the pharmacogenomics of bleomycin, offering insights into how genetic variations among patients may influence their responses to chemotherapy. Overall, the investigation of BLMH at the molecular level represents a promising area of research that could enhance our understanding of not only bleomycin metabolism but also broader drug resistance mechanisms in cancer therapy.
