Analytical Data
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Gene name
ENPP-5
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简介
ENPP-5, also known as ectonucleotide pyrophosphatase/phosphodiesterase 5, has the ability to hydrolyze nicotinamide adenine dinucleotide (NAD) but does not have lysophospholipase D activity, indicating its inability to hydrolyze Nucleotide diphosphates and triphosphates. This enzymatic activity suggests that ENPP-5 may play a role in regulating NAD levels and possibly affecting cellular processes involved in NAD metabolism. ENPP-5 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-5 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
Ectonucleotide pyrophosphatase/phosphodiesterase family member 5; NPP-5
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9UJA9
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Expression Region
P25-G429
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AA Sequence
PDQQKVLLVSFDGFRWDYLYKVPTPHFHYIMKYGVHVKQVTNVFITKTYPNHYTLVTGLFAENHGIVANDMFDPIRNKSFSLDHMNIYDSKFWEEATPIWITNQRAGHTSGAAMWPGTDVKIHKRFPTHYMPYNESVSFEDRVAKIIEWFTSKEPINLGLLYWEDPDDMGHHLGPDSPLMGPVISDIDKKLGYLIQMLKKAKLWNTLNLIITSDHGMTQCSEERLIELDQYLDKDHYTLIDQSPVAAILPKEGKFDEVYEALTHAHPNLTVYKKEDVPERWHYKYNSRIQPIIAVADEGWHILQNKSDDFLLGNHGYDNALADMHPIFLAHGPAFRKNFSKEAMNSTDLYPLLCHLLNITAMPHNGSFWNVQDLLNSAMPRVVPYTQSTILLPGSVKPAEYDQEG
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Protein Length
Partial
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Molecular Weight
68-95 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ENPP-5, also known as ecto-nucleotide pyrophosphatase/phosphodiesterase family member 5, plays a significant role in nucleotide metabolism and cellular signaling processes. Recent studies have indicated that ENPP-5 is involved in tumor development and progression, making it a potential target for cancer therapy. The protein is implicated in the regulation of extracellular ATP and adenosine levels, which are crucial for modulating immune responses and inflammation. Given its involvement in such vital biological functions, researchers have prioritized the production and characterization of recombinant ENPP-5. This involves the use of various expression systems, such as bacteria, yeast, or mammalian cells, to produce sufficient quantities of the protein for in-depth functional studies and structural analyses. By understanding the enzymatic activity and substrate specificity of ENPP-5, researchers aim to elucidate its mechanism of action and its potential as a therapeutic target. Furthermore, the study of ENPP-5's interactions with other molecules may reveal insights into its role in disease pathogenesis, particularly in cancer and other pathological conditions. As a result, the investigation of recombinant ENPP-5 not only contributes to our fundamental understanding of cellular processes but also opens avenues for the development of novel therapeutic strategies aimed at ameliorating diseases linked to dysregulated nucleotide metabolism and signaling.
