Analytical Data
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Gene name
Enolase 3/ENO3
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简介
Enolase 3/ENO3 protein acts as a glycolytic enzyme, promoting the conversion of 2-phosphoglycerate into phosphoenolpyruvate. In addition to its role in glycolysis, ENO3 appears to play a crucial role in the development and regeneration of striated muscle. Enolase 3/ENO3 Protein, Human (His) is the recombinant human-derived Enolase 3/ENO3 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Beta-enolase; Enolase 3; MSE; Skeletal muscle enolase
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Species
Human
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Source
E. coli
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13929-1
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Expression Region
M1-K434
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AA Sequence
MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK
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Protein Length
Full Length of Isoform-1
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Molecular Weight
50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Enolase 3, also known as ENO3, is a key glycolytic enzyme that plays a crucial role in the conversion of 2-phosphoglycerate to phosphoenolpyruvate, facilitating energy production in cells. It exists in multiple isoforms, with ENO3 being predominantly expressed in muscle and brain tissues. Research on ENO3 has garnered attention due to its involvement in various physiological and pathological processes, including muscle glycolytic capacity, cancer metabolism, and neurodegenerative diseases. The study of recombinant ENO3 protein is pivotal for elucidating its biochemical properties, regulatory mechanisms, and interactions with other metabolic pathways. Furthermore, characterizing ENO3 as a potential biomarker or therapeutic target could provide insights into diseases associated with altered energy metabolism. Advances in recombinant DNA technology have enabled the production of ENO3 in heterologous systems, allowing for detailed functional studies and high-throughput screenings for inhibitors or modulators. Understanding this enzyme's structure-function relationship may open new avenues for therapeutic intervention in metabolic disorders and enhance our knowledge of muscle function and plasticity under varying physiological conditions. Overall, the exploration of recombinant ENO3 not only underscores its importance in fundamental biology but also highlights its potential implications in clinical research and therapeutic development.
