Analytical Data
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Gene name
RAC1
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简介
The RAC1 protein is a plasma membrane-associated small GTPase that dynamically regulates multiple cellular responses by cycling between an active GTP-bound state and an inactive GDP-bound state. In its active form, RAC1 regulates processes such as secretory function, phagocytosis, cell polarization, neuronal activity, and growth factor-induced membrane ruffle formation. RAC1 Protein, Human (sf9, GST) is the recombinant human-derived RAC1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Application
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Alternative Names
Ras-related C3 botulinum toxin substrate 1; p21-Rac1; RAC1; TC25; MIG5
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P63000-1
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Expression Region
M1-C189
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Protein Length
Full Length of Isoform 1 Mature Protein
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAC1 is a small GTPase belonging to the Rho family of proteins, playing a crucial role in various cellular processes, including cytoskeletal dynamics, cell migration, and signal transduction. Its function is tightly regulated by the cycling between active (GTP-bound) and inactive (GDP-bound) states. Abnormal RAC1 activity has been implicated in several diseases, particularly cancer, where it contributes to tumor progression and metastasis by promoting cell motility and invasion. The study of RAC1, particularly through the use of recombinant protein techniques, allows researchers to dissect its signaling pathways and interactions with downstream effectors. This recombinant approach facilitates the production of purified RAC1 protein, which can then be utilized in biochemical assays to investigate its functional properties, such as nucleotide exchange, GTP hydrolysis, and interaction with proteins involved in the actin cytoskeleton remodeling. Understanding these mechanisms is essential for developing targeted therapeutic strategies aimed at modulating RAC1 function in pathological conditions. Recent advancements in structural biology have also provided insights into the conformational changes RAC1 undergoes during its activation, further enriching our knowledge of its role in cellular physiology and pathology.
