Analytical Data
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基因名
SMPDL3A
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简介
SMPDL3A protein hydrolyzes nucleotide triphosphates, particularly ATP, and p-nitrophenyl-TMP. It has limited activity with nucleoside diphosphates and none with nucleoside monophosphates. SMPDL3A also catalyzes the conversion of CDP-choline to CMP and phosphocholine, and CDP-ethanolamine. However, it lacks sphingomyelin phosphodiesterase activity. SMPDL3A Protein, Mouse (HEK293, His) is the recombinant mouse-derived SMPDL3A protein, expressed by HEK293 , with C-His labeled tag.
- Application
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别名
Acid sphingomyelinase-like phosphodiesterase 3a; ASM-like phosphodiesterase 3a; SMPDL3A; ASML3A
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种属
Mouse
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P70158
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表达区间
V23-L445
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氨基酸序列
VPLAPADRAPAVGQFWHVTDLHLDPTYHITDDRTKVCASSKGANASNPGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELSTGTVIKVITNMTMTVQNLFPNLQVFPALGNHDYWPQDQLPIVTSKVYSAVADLWKPWLGEEAISTLKKGGFYSQKVASNPGLRIISLNTNLYYGPNIMTLNKTDPANQFEWLENTLNSSLWNKEKVYIIAHVPVGYLPYATDTPAIRQYYNEKLLDIFRRYSSVIAGQFYGHTHRDSLMVLSDKNGNPLNSVFVAPAVTPVKGVLQKETNNPGVRLFQYKPGDYTLLDMVQYYLNLTEANLKGESNWTLEYVLTQAYSVADLQPKSLYALVQQFATKDSKQFLKYYHYYFVSYDSSATCDQHCKTLQVCAIMNLDSMSYDDCLKQHL
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蛋白长度
Full Length of Mature Protein
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分子量
55-75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SMPDL3A (Sphingomyelin Phosphodiesterase Acid-Like 3A) is an enzyme involved in sphingolipid metabolism, particularly in the hydrolysis of sphingomyelin to generate bioactive lipid metabolites. Research into SMPDL3A has gained traction due to its potential roles in various physiological and pathological processes, such as cell signaling, tumor progression, and immune responses. Deficiency or dysregulation of SMPDL3A has been associated with several diseases, including cancer and neurodegenerative disorders, highlighting its importance in maintaining cellular homeostasis. Moreover, the enzyme's activity is crucial for the modulation of sphingolipid pathways, which are increasingly recognized for their involvement in metabolic regulation and cellular communication. As a result, the study of recombinant SMPDL3A proteins has become essential for understanding its enzymatic functions and regulatory mechanisms. This research not only aims to elucidate the biological significance of SMPDL3A but also explores its potential as a therapeutic target. By generating and analyzing recombinant forms of SMPDL3A, scientists can dissect its role within cellular contexts, providing insights into how it may be manipulated for future clinical applications. Overall, the exploration of SMPDL3A and its recombinant proteins stands at the intersection of biochemistry and clinical research, seeking to translate basic scientific findings into innovative strategies for disease intervention.
