Analytical Data
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Gene name
Enolase 1/ENO1
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简介
The ENO1 protein converts 2-phosphoglycerate to phosphoenolpyruvate and is involved in growth control, hypoxia tolerance, and allergic responses. It acts as a receptor and activator of plasminogen, stimulating immunoglobulin production. ENO1 binds to the myc promoter as a transcriptional repressor and may function as a tumor suppressor. Enolase 1/ENO1 Protein, Human (His, solution) is the recombinant human-derived ENO1, expressed by E. coli , with C-His labeled tag.
- Application
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Alternative Names
rHuAlpha-enolase, His; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein; ENO1
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06733-1
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Expression Region
M1-K434
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AA Sequence
MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
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Protein Length
Full Length of Isoform-1
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Molecular Weight
45-55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Enolase 1 (ENO1), also known as alpha-enolase, is a glycolytic enzyme that plays a crucial role in carbohydrate metabolism by catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate, a key step in the glycolytic pathway. Beyond its metabolic functions, ENO1 is implicated in various cellular processes, including cell proliferation, differentiation, and response to stress. Research has shown that ENO1 is overexpressed in numerous cancers, suggesting a potential role in tumorigenesis and cancer progression, making it a target of interest for cancer diagnostics and therapeutics. Moreover, ENO1 exhibits moonlighting properties, functioning also as a moonlighting protein in processes such as immune responses and cytoskeletal organization. The production of recombinant ENO1 protein has facilitated the exploration of its structure-function relationships, interactions with other proteins, and mechanistic insights into its dual roles in metabolism and signaling pathways. Understanding the biological implications of ENO1 can not only enhance our knowledge of metabolic regulation but also pave the way for novel therapeutic strategies, particularly in oncology. As researchers continue to investigate ENO1's diverse functions and therapeutic potentials, the production and characterization of ENO1 recombinant protein remain essential for unveiling its roles in health and disease.
