Analytical Data
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Gene name
USH1C
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简介
USH1C is an anchoring and scaffolding protein critical for cochlear hair cell mechanotransduction. It forms a network with USH1G, CDH23 and MYO7A and contributes to the development and maintenance of hair cell bundles. USH1C Protein, Human (His) is the recombinant human-derived USH1C protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Harmonin; Antigen NY-CO-38/NY-CO-37; Protein PDZ-73; AIE75
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9Y6N9
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Expression Region
M1-F552
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AA Sequence
MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEAVNVLKSSRSLTISIVAAAGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKYDQGVEPELEPADDLDGGTEEQGEQDFRKYEEGFDPYSMFTPEQIMGKDVRLLRIKKEGSLDLALEGGVDSPIGKVVVSAVYERGAAERHGGIVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELTFF
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Protein Length
Full Length
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Molecular Weight
70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USH1C, a gene associated with Usher syndrome type 1C, encodes the protein harmonin, which plays a crucial role in the development and function of auditory and vestibular systems as well as photoreceptor cells in the retina. Mutations in USH1C are linked to the combined hearing loss and vision impairment characteristic of this syndrome. Research into USH1C recombinant proteins is essential for understanding the molecular mechanisms underlying these sensory deficits, facilitating the exploration of therapeutic avenues. The study of harmonin's structure and function can reveal its interactions with other proteins involved in cell signaling and sensory transduction pathways. Additionally, the expression and purification of USH1C recombinant proteins enable the development of assays for drug screening, potential gene therapy strategies, and the investigation of its roles in cellular processes, such as cell adhesion and cytoskeletal organization. As advancements in biotechnology continue, the characterization of USH1C and its protein products holds promise for targeted interventions that could mitigate the effects of Usher syndrome, improve patient outcomes, and contribute to a greater understanding of related auditory and visual disorders. Furthermore, these studies can pave the way for novel insights into the genetic and environmental factors that influence sensory cell function and contribute to the overall health of hearing and vision.
