Analytical Data
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Gene name
CBFB
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简介
CBFB forms a heterodimeric complex core binding factor (CBF) with RUNX family proteins, which enhances the DNA binding ability of RUNX members (such as RUNX1, RUNX2, and RUNX3). Heterodimers bind to specific sites in enhancers and promoters to regulate the transcription of target genes. CBFB Protein, Human (His) is the recombinant human-derived CBFB protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Core-binding factor subunit beta; CBF-beta; PEA2-beta; PEBP2-beta
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q13951-1
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Expression Region
P2-P182
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AA Sequence
PRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTTRP
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Protein Length
Partial
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CBFB (core-binding factor beta) is a critical component of the core-binding factor (CBF) complex, which plays an essential role in hematopoiesis and development. The CBFB gene encodes a transcription factor that partners with RUNX proteins to regulate the expression of genes involved in cell differentiation, proliferation, and survival. Dysregulation of CBFB has been implicated in various hematological malignancies, particularly acute myeloid leukemia (AML). CBFB rearrangements, such as CBFB-MYH11 fusion, have been shown to disrupt normal hematopoietic development and contribute to leukemogenesis, making it a significant target for therapeutic intervention. Researchers have focused on characterizing the structure and function of CBFB as well as developing recombinant protein forms for functional studies. These studies aim to elucidate the mechanisms by which CBFB influences gene expression and cellular behavior, providing insights into its role in cancer biology. Understanding the biology of CBFB and its interactions can pave the way for targeted therapies that may improve treatment outcomes for patients with malignancies associated with CBFB abnormalities. The development of recombinantly expressed CBFB proteins has also enabled high-throughput screening of small molecules that may inhibit its oncogenic functions, further contributing to the potential for novel therapeutic strategies.
