Analytical Data
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Gene name
Fructose-bisphosphate aldolase A/ALDOA
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简介
Fructose-bisphosphate aldolase A (ALDOA) is a key enzyme that catalyzes the reversible conversion of β-D-fructose 1,6-bisphosphate (FBP) into two triose phosphates in glycolysis and gluconeogenesis. play a central role. By mediating this critical step in carbohydrate metabolism, ALDOA helps produce energy and metabolic intermediates necessary for cellular function. Fructose-bisphosphate aldolase A/ALDOA Protein, Human (His) is the recombinant human-derived Fructose-bisphosphate aldolase A/ALDOA protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Lung cancer antigen NY-LU-1, Muscle-type aldolase
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P04075
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Expression Region
P2-Y364
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AA Sequence
MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY
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Protein Length
Full Length of Mature Protein
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Molecular Weight
40-46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Fructose-bisphosphate aldolase A (ALDOA) is a key enzyme in the glycolytic pathway, facilitating the reversible cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. This enzyme plays a crucial role in energy metabolism and is essential for maintaining cellular homeostasis. Dysregulation of ALDOA has been implicated in several diseases, including cancer, where altered glycolytic metabolism supports rapid cell proliferation. In recent years, studies have highlighted the potential of ALDOA as a therapeutic target and biomarker for various conditions. The expression and functional characterization of recombinant ALDOA provide valuable insights into its enzymatic mechanisms and interaction with other metabolic pathways. Furthermore, the production of recombinant ALDOA allows for the exploration of its structural properties, which can aid in the design of specific inhibitors or modulators. Understanding ALDOA at the molecular level is vital for elucidating its role in metabolic disorders and developing innovative therapeutic strategies, thereby underscoring the importance of research in this area.
