Recombinant Human SMOC1 Protein (HEK293)，His

SMOC1

SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 Protein, Human (HEK293, His) is the recombinant human-derived SMOC1 protein, expressed by HEK293 , with C-8*His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37°C. The ED50 for this effect is 0.5-1.5 μg/mL. Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37°C. The ED50 for this effect is 0.5833 μg/mL, corresponding to a specific activity is 1714.384 units/mg.

SPARC-related modular calcium-binding protein 1; SMOC-1

Human

HEK293

C-8*His

Greater than 95% as determined by SDS-PAGE.

Q9H4F8-2

H27-V435

HRTTGPRFLISDRDPQCNLHCSRTQPKPICASDGRSYESMCEYQRAKCRDPTLGVVHRGRCKDAGQSKCRLERAQALEQAKKPQEAVFVPECGEDGSFTQVQCHTYTGYCWCVTPDGKPISGSSVQNKTPVCSGSVTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNIRNSEKVYSCDQERQSALEEAQQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTGRPLPGTSTRYVMPSCESDARAKTTEADDPFKDRELPGCPEGKKMEFITSLLDALTTDMVQAINSAAPTGGGRFSEPDPSHTLEERVVHWYFSQLDSNSSNDINKREMKPFKRYVKKKAKPKKCARRFTDYCDLNKDKVISLPELKGCLGVSKEVGRLV

Partial

55-60 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.