Analytical Data
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Gene name
AACS
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简介
AACS proteins play a key role in cellular metabolism by converting acetoacetate in the cytoplasm into acetoacetyl-CoA (by similarity). As a ketone body utilizing enzyme, AACS helps synthesize essential cellular components including cholesterol and fatty acids (by similarity). AACS Protein, Human (sf9, His) is the recombinant human-derived AACS protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Acetoacetyl-CoA synthetase; AACS; ACSF1
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Species
Human
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86V21-1
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Expression Region
M1-F672
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Protein Length
Full Length of Isoform-1
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of AACS (Acetoacetyl-CoA synthetase) recombinant protein has gained significance due to its crucial role in numerous metabolic pathways, particularly in fatty acid metabolism and the synthesis of ketone bodies. AACS catalyzes the activation of acetoacetate, facilitating its conversion into acetoacetyl-CoA, a key intermediate in the synthesis of cholesterol and fatty acids. Understanding the structure and function of AACS can provide insights into metabolic disorders, such as obesity and diabetes, where fatty acid metabolism is disrupted. Moreover, recombinant technology enables the production of AACS in large quantities, allowing for detailed biochemical analyses and potential therapeutic applications. The exploration of AACS also enhances our understanding of the evolutionary adaptations in various organisms, as different species exhibit varied enzymatic efficiency and regulatory mechanisms. Consequently, research on AACS recombinant protein not only contributes to basic metabolic biology but also holds promise for innovative approaches in biotechnology and medicine, aiming to manipulate metabolic pathways for health benefits.
