Analytical Data
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基因名
AACS
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简介
AACS proteins play a key role in cellular metabolism by converting acetoacetate in the cytoplasm into acetoacetyl-CoA (by similarity). As a ketone body utilizing enzyme, AACS helps synthesize essential cellular components including cholesterol and fatty acids (by similarity). AACS Protein, Human (sf9, His) is the recombinant human-derived AACS protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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别名
Acetoacetyl-CoA synthetase; AACS; ACSF1
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种属
Human
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表达系统
Baculovirus
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86V21-1
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表达区间
M1-F672
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蛋白长度
Full Length of Isoform-1
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分子量
60 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of AACS (Acetoacetyl-CoA synthetase) recombinant protein has gained significance due to its crucial role in numerous metabolic pathways, particularly in fatty acid metabolism and the synthesis of ketone bodies. AACS catalyzes the activation of acetoacetate, facilitating its conversion into acetoacetyl-CoA, a key intermediate in the synthesis of cholesterol and fatty acids. Understanding the structure and function of AACS can provide insights into metabolic disorders, such as obesity and diabetes, where fatty acid metabolism is disrupted. Moreover, recombinant technology enables the production of AACS in large quantities, allowing for detailed biochemical analyses and potential therapeutic applications. The exploration of AACS also enhances our understanding of the evolutionary adaptations in various organisms, as different species exhibit varied enzymatic efficiency and regulatory mechanisms. Consequently, research on AACS recombinant protein not only contributes to basic metabolic biology but also holds promise for innovative approaches in biotechnology and medicine, aiming to manipulate metabolic pathways for health benefits.
