Analytical Data
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Gene name
MAGOH
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简介
MAGOH is an important spliceosome component that cooperates with MAGOHB to form the exon junction complex (EJC), which is central to pre-mRNA splicing and nonsense-mediated decay (NMD). EJC affects mRNA metabolism, marks exon-exon junctions, and affects processes such as mRNA export, localization, translation, and NMD. MAGOH Protein, Human (His) is the recombinant human-derived MAGOH protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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Alternative Names
Protein mago nashi homolog; MAGOH; MAGOHA
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P61326-1
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Expression Region
M1-I146
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Protein Length
Full Length of Isoform-1
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Molecular Weight
17 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAGOH, a key component of the exon junction complex (EJC), plays a crucial role in RNA metabolism, including mRNA stability, splicing, and translation regulation. This protein interacts with various RNA-binding proteins and is implicated in several cellular processes, such as gene expression regulation and response to cellular stress. Recent studies have highlighted the importance of MAGOH in cancer biology, demonstrating its involvement in tumor progression and metastasis. Additionally, MAGOH has been linked to the modulation of RNA processing and surveillance mechanisms, suggesting that its dysregulation can lead to various diseases, including neurodegenerative disorders. The exploration of MAGOH's structure and function through recombinant protein studies is therefore critical for understanding its biological roles and potential as a therapeutic target. Researchers aim to investigate the biochemical properties of MAGOH, its interactions with other EJC components, and its influence on mRNA fate, which can provide insights into new avenues for treatment in diseases where MAGOH is deregulated. By producing and characterizing recombinant MAGOH, scientists hope to elucidate its mechanisms of action and develop strategies for modulating its function in a clinical context.
