Analytical Data
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Gene name
INPP5A
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简介
INPP5A Protein, a phosphatase, catalyzes the hydrolysis of inositol phosphates, converting inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. This enzymatic action underscores its role in inositol phosphate metabolism. INPP5A is crucial for the survival of cerebellar Purkinje cells, emphasizing its significance in cellular processes and neuronal function. INPP5A Protein, Human (HEK293, His) is the recombinant human-derived INPP5A protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
Type I inositol 1; 4; 5-trisphosphate 5-phosphatase; INPP5A
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14642
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Expression Region
M1-V410
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AA Sequence
MAGKAAAPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHTHKPHFMALHCQEFGGKNYEASMSHVDKFVKELLSSDAMKEYNRARVYLDENYKSQEHFTALGSFYFLHESLKNIYQFDFKAKKYRKVAGKEIYSDTLESTPMLEKEKFPQDYFPECKWSRKGFIRTRWCIADCAFDLVNIHLFHDASNLVAWETSPSVYSGIRHKALGYVLDRIIDQRFEKVSYFVFGDFNFRLDSKSVVETLCTKATMQTVRAADTNEVVKLIFRESDNDRKVMLQLEKKLFDYFNQEVFRDNNGTALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDRILMSPSAKELVLRSESEEKVVTYDHIGPNVCMGDHKPVFLAFRIMPGAGKPHAHVHKCCV
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Protein Length
Partial
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Molecular Weight
45-48 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
INPP5A (Inositol Polyphosphate Phosphatase A) is an important enzyme involved in the phosphoinositide signaling pathway, which plays a critical role in various cellular processes, including cell growth, differentiation, and metabolism. Research has increasingly focused on exploring the functional implications of INPP5A due to its involvement in regulating intracellular calcium levels and its potential role in cancer biology and neurodegenerative diseases. Elevated levels of INPP5A have been associated with tumor progression and poor prognosis in several cancer types, highlighting its potential as a therapeutic target. Moreover, the recruitment of INPP5A to specific cellular membranes and its interaction with other signaling molecules suggest that this enzyme could serve as a crucial regulator of cellular signaling networks. The study of recombinant INPP5A protein is vital for understanding its biochemical properties, interactions, and physiological functions in detail. By producing and purifying INPP5A in a recombinant system, researchers can investigate its enzymatic activity, structural characteristics, and dynamic regulation in response to different stimuli. This knowledge may lead to the development of novel therapeutic strategies aimed at modulating INPP5A activity to treat various diseases, particularly those where dysregulation of phosphoinositide signaling is implicated. Overall, INPP5A represents a significant focus in cellular signaling research, and its recombinant protein studies hold promise for advancing our understanding of its role in health and disease.
