Analytical Data
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基因名
NETO1
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简介
The NETO1 protein complexly maintains neuronal circuits as an auxiliary subunit of NMDARs, which is critical for the abundance of GRIN2A-containing NMDARs in the postsynaptic density. It regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in spatial learning and memory. NETO1 Protein, Human (HEK293, His) is the recombinant human-derived NETO1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED50 of 0.6861 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED50 of 0.6861 μg/mL.
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别名
Neuropilin and tolloid-like protein 1; NETO1; BTCL1
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q8TDF5-3
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表达区间
T23-T344
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氨基酸序列
TKKGTEKQTTSETQKSVQCGTWTKHAEGGIFTSPNYPSKYPPDRECIYIIEAAPRQCIELYFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPVIKSSGRFLWIKFFADGELESMGFSARYNFTPDPDFKDLGALKPLPACEFEMGGSEGIVESIQIMKEGKATASEAVDCKWYIRAPPRSKIYLRFLDYEMQNSNECKRNFVAVYDGSSSVEDLKAKFCSTVANDVMLRTGLGVIRMWADEGSRNSRFQMLFTSFQEPPCEGNTFFCHSNMCINNTLVCNGLQNCVYPWDENHCKEKRKTSLLDQLTNTSGT
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蛋白长度
Extracellular Domain
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分子量
40-50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NETO1 (Neurotrophin-Dependent Endosomal Transporter 1) is a protein that plays a significant role in the neurotrophic signaling pathways, particularly in synaptic plasticity and neuronal survival. Research on NETO1 has gained momentum due to its involvement in the transport and signaling of neurotrophins, which are crucial for the development and maintenance of the nervous system. Abnormalities in NETO1 expression or function have been linked to neurodevelopmental disorders and neurodegenerative diseases, making it a potential target for therapeutic interventions. The understanding of NETO1's structure and its molecular interactions can provide insights into its role in neuronal health and disease. Recent studies have employed recombinant protein techniques to investigate the functional properties of NETO1, aiming to elucidate its mechanism of action at the cellular level. This research is particularly relevant for developing strategies to modulate NETO1 activity in conditions where neurotrophin signaling is disrupted. Overall, the investigation of NETO1 and its recombinant protein is pivotal in advancing our knowledge of neuronal function and may contribute to innovative treatments for various neurological disorders.
