Analytical Data
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Gene name
KIAA0101
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简介
The KIAA0101 protein is a PCNA-binding regulator that plays a key role in DNA repair during replication. After DNA damage, it disrupts its interaction with PCNA and promotes the binding between monoubiquitinated PCNA and DNA polymerase eta (POLH). KIAA0101 Protein, Human (His) is the recombinant human-derived KIAA0101 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
PCNA-associated factor; OEATC-1; PAF15; p15PAF; PCLAF; KIAA0101; NS5ATP9; PAF
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q15004-1
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Expression Region
M1-E111
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Protein Length
Full Length of Isoform-1
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Molecular Weight
17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KIAA0101, also known as TIPIN (TIM-associated protein interacting with the PCNA), is a protein that plays a crucial role in DNA replication and repair processes. It associates with the proliferating cell nuclear antigen (PCNA), a key protein involved in DNA synthesis and repair, thereby influencing the stability and efficiency of the replication fork. Understanding KIAA0101 is important because it has been implicated in cellular responses to DNA damage and has a potential role in cancer biology. Abnormal expression of KIAA0101 has been linked to various cancers, suggesting that it may serve as a biomarker for tumor progression or a target for therapeutic interventions. Recent studies have focused on elucidating its molecular mechanisms, including its interactions with other proteins involved in DNA damage response pathways. Furthermore, researchers have explored KIAA0101's potential role in modulating replication stress, a condition often observed in cancer cells due to uncontrolled proliferation. Investigating KIAA0101's function not only enhances our understanding of fundamental cellular processes but also opens up avenues for developing novel cancer treatments by targeting its interactions or modulating its activity. Overall, the ongoing research into KIAA0101 is expected to contribute significantly to both basic biology and clinical applications in oncology.
